If this is a mutant or different space group or in complex, which residues have changed conformation?

Difference Distance Matrix Plot (DDMP) may be used to answer the question of which regions of the backbone are different. Below is the full output of a DDMP comparison of the A and B monomers in the pyrococcus woesei TATA-binding crystal structure. It is obvious that the major backbone differences are in the region of residues 33 - 39 with slight changes around residues 95 and 125.

The large changes in the region of residues 34-39 can be seen in a superpositioned backbone image using MIDAS:

But the smaller changes observed by DDMP in other regions might be missed by this approach.

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Revised: Tuesday, 20-Oct-1998 14:49:50 EDT