**************************************************************** XALIGN Parameters **************************************************************** Enter the file which contains the amino acid similarity scoring matrix and the various gap penalties. This file contains a lower triangular matrix which indicates the similarity score between two amino acids. The matrix is of a general nature and may not reflect the specific needs of the user (for example, a user who finds that cystines are not lining up with cystines may want to increase the score for such a match). XALIGN:SCOR_MATRIX $INSTALL/lib/xalign/wtm.rbo ---------------------------------------------------------------- Indicate if pairwise alignments should be printed. This can greatly increase the output however it often contains useful information. Enter "1" for printing and "0" for not printing. XALIGN:PRINT_PAIRWISE 0 ---------------------------------------------------------------- The following is an offset added to the percent homology score of a pairwise alignment if secondary structure is known for a given sequence. In general, any sequence which has secondary structure known should be given precedence over those that do not when determining the order of sequences in the multiple alignment. An offset of "0" means the order of the sequences in the multiple alignment algorithm is based only on primary sequence homology. Range: MIN_STRUCT_BIAS <= x <= MAX_STRUCT_BIAS XALIGN:STRUCT_BIAS 100 ---------------------------------------------------------------- As more and more sequences are added to the multiple alignment, the cumulative effect of all the amino acid scoring starts to dwarf the gapping penalties. Therefore we probably want to increase the gap and gap size penalties by a given percent for each sequence added. For example, this number should be adjusted up if too many gaps are found in the final sequences of the multiple alignment. Range: MIN_INC_GAP_PEN <= x <= MAX_INC_GAP_PEN XALIGN:INC_GAP_PEN 30 ---------------------------------------------------------------- Indicate if the pairwise sequence percent homology table should be printed. This table allows the user to quickly compare pairwise sequence alignments in terms of percent homology. Enter "0" for not printing and "1" for printing. Range: 0 <= x <= 1 XALIGN:PRINT_PCT_HOM 1 ---------------------------------------------------------------- Indicate the order of the sequences in your multiple alignment output. To have sequences printed in the order that the program ranks them (for adding them to the multiple alignment), enter "0". If you prefer to have the sequences printed in the order that they were entered into the program, enter "1". Range: 0 <= x <= 1 XALIGN:PRINT_ORDER 0 ---------------------------------------------------------------- Following the multiple alignment, a consensus sequence is printed. At what percent identity is a consensus reached for a given amino acid at a given position? Enter an integer from 0 - 100 to represent this threshold. Range: 0 <= x <= 100 XALIGN:CONSENSUS_PCT 70 ---------------------------------------------------------------- Do not change these parameters, they were added for the sake of compatibility with other packages that use the low level xalign engine. XALIGN:DATA_FORMAT 1 XALIGN:DATA_FILE x XALIGN:SEQ_SELECT 1